Category Archives: pda agar

pda agar

Grow mushroom cultures from spore

mycelium running

If you purchased a spore print or a culture syringe to start your mushroom cultivation, your next step would be to prepare AGAR media and pour that into Petri dishes.. Agar is a seaweed extract, a gelatinous substance that will solidify at room temperature. It can be sterilized by pressure and is the ideal food […]

Preparing Agar (PDA)

PDA Culture Media Dishes

Preparing your Agar For every 1 Litre of Agar Final Mixed Solution you will need the following. 20 grams Bacteriological Agar 200g Potato infusion 4 g of Malt Extract or Yeast Extract (optional) 20 g Dextrose 1 Litre of Distilled Water Autoclave or Pressure Vessel Method: Place your cleaned Erlenmeyer flask on a kitchen scale […]

Cultivate Shiitake Mushrooms

cultivating shiitake mushrooms

A) Shiitake from Spore If you purchased a spore print or a culture syringe to start your shiitake (also known as Golden Oak Mushroom) cultivation, your next step would be to learn how to prepare AGAR media. Agar is a seaweed extract, a gelatinous substance that will solidify at room temperature. Sterilized by pressure cooking […]

Culturing Wild Mushrooms

During the autumn of 2014, I harvested and cloned a mushroom on my Farm that in my opinion looked much like the Pleurotus ostreatus strain (Oyster Mushroom). Looking at the culture running on PDA (potatoe dextrose agar) in a petri dish showed all the same characteristics as the other Pleurotus cultures I work with. Some […]